Product Number: PLK0301, PLK0302 PLK0303
Shipping and Storage
- RNase A is stored in a ready to use glycerol buffer and transported at room temperature. Upon receipt, it should be stored at room temperature not exceeding 25 ℃ for at least 6 months, at 4 ℃ for 12 months, and for long-term storage at -20 ℃.
- When using for the first time, add all RNase A from the test sample to Buffer P1 (final concentration 100μg/ml) and store at 2-8℃. If RNase A is inactivated in Buffer P1, there may be trace RNA residue in the extracted plasmid. Adding RNase A to Buffer P1 is sufficient.
- When the ambient temperature is low, SDS in Buffer P2 may precipitate turbidity or sediment. It can be heated in a 37 ℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.
Components
| Component | Storage | PLK0301 50 Preps | PLK0302 100 Preps | PLK0303 200 Preps |
| Balance Buffer | RT | 5 ml | 10ml | 20 ml |
| RNaseA (10mg/ml) | 4°C | 150 µl | 250μl | 500 µl |
| Buffer P1 | 4°C | 15 ml | 25ml | 50 ml |
| Buffer P2 | RT | 15 ml | 25ml | 50 ml |
| Buffer P3 | RT | 20 ml | 35ml | 70 ml |
| Buffer PE | RT | 16 ml | 31.5ml | 64 ml |
| Buffer WB | RT | 13 ml | 25ml | 50 ml |
| Buffer EB | RT | 10 ml | 15ml | 20 ml |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube(2ml) | RT | 50 | 100 | 200 |
Note:Buffer PE、Buffer WB-Add the specified amount of ethanol according to the instructions before the first use.
Description
This reagent kit uses an improved SDS alkaline lysis method to lyse cells. The silicon matrix membrane in the centrifuge adsorption column selectively binds to plasmid DNA in the solution under high salt and low pH conditions. Then, impurities and other bacterial components are removed through Buffer PE and Buffer WB. Finally, the pure plasmid DNA is eluted from the silicon matrix membrane with low salt and high pH Buffer EB
Features
- The silicon matrix membranes inside the centrifugal adsorption column are all made by imported world-renowned companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- The unique de Buffer PE formula can efficiently remove residual nucleases, even for strains with rich nuclease content such as JM series and HB101, it can be easily removed. Effectively preventing plasmid degradation by nuclease.
- It is fast and convenient, and does not require the use of toxic reagents such as phenol and chloroform, nor does it require ethanol precipitation. The obtained plasmids have high yield and good purity, and can be directly used for various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
