Manual

Product Number: DNK77MB

Description

This kit uses magnetic beads with unique separation capabilities and a unique buffer system to isolate and purify high-quality DNA from various cultured and parasitic fungal samples. The reagent kit undergoes steps such as magnetic bead binding with nucleic acid, cleaning, and elution to remove protein and other impurities. The resulting DNA product has high purity and can be directly applied to various downstream molecular biology experiments such as qPCR, library construction, NGS, etc. This product can be perfectly matched with an automatic nucleic acid extractor. By adsorbing, transferring, and releasing magnetic beads with a magnetic rod, the transfer of magnetic beads and nucleic acids can be achieved, improving the degree of automation. The entire experimental process is safe and convenient, and the extracted DNA has high purity without contamination from proteins and other impurities.

Application

This product is suitable for rapid extraction of DNA from various cultured fungal and parasitic fungal samples, and the processed products can be used for scientific research. This product is only for scientific research purposes and is used for the extraction, enrichment, and purification steps of nucleic acids.

Components

Pre packaged: 32 reaction/box, 48 reaction/box, 64 reaction/box (note: 8-reaction or 16 reaction pre packaged reagent plates can be selected)

Component	Specifications
	32 Reaction/Box	48 Reaction/Box	64 Reaction/Box
8 Reaction pre packaged reagent board	4 pieces	6 pieces	8 pieces
16 Reaction pre packaged reagent board	2 pieces	3 pieces	4 pieces
8 Joint magnetic rod cover	4 pieces	6 pieces	8 pieces
SACP liquid	22mL/Bottle	32mLBottle	42mL/Bottle
NSH liquid	7mL/Bottle	10.5mL/Bottle	13.5mL/Bottle
Eluent	2mL/Tube	2mL/Tube	2mL/Tube
SURBEAD	1 Reaction/Tube*32	1Reaction/Tub*48	1Reaction/Tub*64
Settling agent	1.1mL/Tube	1.8mL/Tube	1.1mL/Tube*2
Protease K solution	0.7mL/Tube	1.2mLTube	1.4mL/Tube
RNase A	0.4mL/Tube	0.6mL/Tube	0.7mL/Tube

Pre packaged: 96 reactions per box

Component	Specifications	Quantity
Combination liquid pre installation plate	96 Reaction/Plate	1piece
Magnetic bead washing solution pre installed plate	96 Reaction/Plate	1piece
Washing solution 1 pre installed plate	96 Reaction/Plate	2pieces
Washing solution 2 pre installed plate	96 Reaction/Plate	1piece
Pre loaded eluent plate	96 Reaction/Plate	1piece
SACP liquid	63mL/Bottle	1Bottle
NSH liquid	21mL/Bottle	1Bottle
SURBEAD	1 Reaction/Tube	96Tubes
Eluent	2mL/Tube	1Tube
96 hole magnetic rod sleeve	96 Reaction/Plate	1piece
Settling agent	3.1mL/Bottle	1Bottle
Protease K solution	1.2mL/Tube	2Tubes
RNase A	1.1mL/Tube	1Tube

Large packaging: 50 reactions per box

Component	Specifications	Quantity
Binding Buffer	33mL/Bottle	1Bottle
Magnetic bead solution	1.2mL/Tube	1Tube
Washing solution 1	75mL/Bottle	1Bottle
Washing solution 2	75mL/Bottle	1Bottle
Eluent	12mL/Bottle	1Bottle
SACP liquid	33mL/Bottle	1Bottle
NSH 液	12mL/Bottle	1Bottle
NSH liquid	1.8mL/Tube	1Tube
SURBEAD	1Reaction/Tube	50Tubes
Protease K solution	1.2mL/Tube	1Tube
RNase A	0.6mL/Tube	1Tube

Note: The components of different types of reagent kits cannot be interchanged, and the components of different batch numbers of reagent kits cannot be interchanged. In order to minimize the impact of the absorbance of the blank solution, the UV absorbance method is used to determine the nucleic acid concentration (A260, A280, A230) using eluent as the blank control.

Storage conditions and expiration date

1. The reagent kit is stored at 4~30℃ and has an expiration date of 18 months.
2. If there is precipitation in the SACP solution, it can be preheated in a 37℃ water bath for 10 minutes before use to dissolve the precipitate without affecting the effect.
3. After opening, store NSH, precipitant, and proteinase K solution at 4℃.

Sample requirements

Applicable specimen types: various cultured fungal and parasitic fungal samples.

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