Product Number: DRK78100
Shipping and Storage
The reagent kit is stored at 4~30℃ and has an expiration date of 18 months.
Components
| Component | DRK78100100Preps | DRK785050Preps |
| Magnetic Beads | 1.7 mL | 0.85 mL |
| Buffer LY | 64 mL | 32 mL |
| Buffer WB1 | 54 mL | 27 mL |
| Buffer WB2 | 126 mL | 2 63 mL |
| Buffer EB | 16 mL | 8 mL |
| LP Buffer | 50 mL | 25 mL |
| Buffer EH2 | 60μl | 30μl |
Note:The components of different models of test kits cannot be interchanged, and the components of test kits with different batch numbers cannot be interchanged. In order to minimize the absorbance effect of blank solution, when measuring nucleic acid concentration (A260, A280, A230) using UV absorbance method, Buffer EB is used as the blank control.
Description
This reagent kit uses magnetic beads with unique separation properties and a unique buffer system to isolate and purify high-quality nucleic acids from plant tissue samples. The reagent kit undergoes a unique nucleic acid extraction solution and Enhancement Buffer 2 pre-treatment, followed by magnetic bead binding, cleaning, and elution steps to remove proteins and other impurities. The resulting nucleic acid product has high purity and can be directly applied in various downstream molecular biology experiments such as qPCR, library construction, and NGS.
This product can be perfectly matched with an automatic nucleic acid extractor. By using specially designed magnetic rods to adsorb, transfer, and release magnetic beads, the transfer of magnetic beads and nucleic acids is achieved, improving the degree of automation. The entire experimental process is safe and convenient, and the extracted nucleic acid has high purity, without protein and other impurities pollution.
Application
This product is suitable for isolating and purifying high-quality nucleic acids from plant tissue samples, and the processed products are used for scientific research.
Sample requirements
1.Applicable sample type: Plant tissue tender leaf samples.
2.Sample storage and transportation: Samples can be used for testing immediately or stored at -20±5℃ for testing. Sample transportation is done by curling at 0℃.
Applicable instruments
A strip nucleic acid extractor with heating in column 6 or a plate nucleic acid extractor with heating in plate 6.
Note
1.Please read this manual carefully before the experiment.
2.Different models of nucleic acid extractors may require different extraction programs due to hardware limitations. Please consult our company for detailed parameters.
3.To avoid any potential biological hazards in the sample, the test sample should be considered infectious and avoid contact with skin and mucous membranes; The handling of samples is recommended to be carried out in a biosafety cabinet that can prevent the outflow of mist. The test tubes and suction heads used in the sample preparation area must be placed in containers containing disinfectants and sterilized together with the waste before being discarded; Sample operation and processing must comply with relevant regulatory requirements: the General Guidelines for Biological Safety in Microbial Biomedical Laboratories and the Regulations on Medical Waste Management issued by the Ministry of Health.
4.The components in the reagent kit must be used within the validity period. Failure to use the components provided in this reagent kit for experiments may result in incorrect results.
5.Laboratory management should strictly follow the management standards of PCR gene amplification laboratories. Experimental personnel must receive professional training, and the experimental process should be strictly divided into zones (reagent preparation zone, sample preparation zone, amplification and product analysis zone). Consumables used should be sterilized and used in one go. Specialized instruments and equipment should be used in each stage of the experimental operation, and supplies in each zone and stage should not be used interchangeably.
6.Use disposable centrifuge tubes and pipettes sterilized under high pressure or purchase centrifuge tubes and pipettes without DNA/RNA enzymes.
7.After completing the nucleic acid extraction of the sample, it is recommended to proceed to the next step of the experiment immediately. Otherwise, please store it at -20℃ for use (within 24 hours).
8.After the experiment is completed, treat the workbench and pipette with 5% hypochlorous acid or 75% alcohol, and then irradiate with a UV lamp for 20-30 minutes.
