Urine DNA Kit
2024-12-05Plant Max Genomic DNA Kit
2024-12-05Marine animal Tussie DNA Kit
Product Number: DNK3701
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Proteinase K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK3701 50 Preps | DNK3701 100 Preps | DNK3701 200 Preps |
| Buffer SL | RT | 11 ml | 20 ml | 40 ml |
| Buffer CB | RT | 11 ml | 20 ml | 40 ml |
| Buffer IR | RT | 25 ml | 50 ml | 100 ml |
| Buffer WB | RT | 13 ml | 25 ml | 50 ml |
| Buffer EB | RT | 15 ml | 15 ml | 15ml×2 |
| Proteinase K | 4℃ | 1 ml | 1ml×2 | 1ml×4 |
| Adsorption column AC | RT | 50 | 100 | 200 |
| Collection tube (2ml) | RT | 50 | 100 | 200 |
Description
The unique Buffer CB/Proteinase K rapidly cleaves cells and inactivates intracellular nucleases, and then selectively adsorbs genomic DNA onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer IR and Buffer WB remove impurities such as cell metabolites and proteins. Finally, low salt Buffer EB elutes pure genomic DNA from the silica matrix membrane.
Application
Suitable for rapid extraction of genomic DNA from various marine animal tissues
