Manual

Product Number: RNK2401

Shipping and Storage

1. After adding anhydrous ethanol to Buffer WS1 and Buffer WS2/3, they can be stored at room temperature for one month. If they need to be stored for a longer period of time, please store them at 4℃. However, before use, they should be restored to room temperature first
2. Buffer WS2/3 may precipitate crystals after using ethanol for a few days, which does not affect its use. Simply do not absorb the crystals and use the supernatant.
3. Transportation is carried out at room temperature without affecting the effectiveness of use
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

In recent years, extensive research on RNA interference and regulatory small RNAs urgently requires a reagent kit that can effectively extract RNA of around 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover small molecule RNA, and phenol/guanidine extraction and ethanol precipitation cannot effectively precipitate and recover small molecule RNA. For blood samples, it is even more difficult to extract due to its own characteristics. This reagent kit uses a unique Buffer LY to rapidly and directly lyse whole blood (liquid samples) and inactivate cell RNA enzymes, with strong organic extraction to remove proteins and DNA. RNA, including small molecule RNA, is adsorbed onto a special silicon-based membrane in a centrifuge column. Then, through a series of rapid rinsing centrifugation steps, the rinse solution further removes impurities such as cell metabolites and proteins. Finally, low salt elution solution elutes pure RNA from the silicon-based membrane.

Features

1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. It also does not require steps such as ethanol precipitation that are prone to losing small RNA molecules.
3. The unique Buffer LY formula can directly lyse whole blood without the need to first lyse and remove red blood cells.
4. Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.0 and minimal DNA residue, which can be used for RNAi, RT-PCR, Northern blot, and various experiments.

Application

Suitable for rapid extraction of miRNAs and other small RNAs from various whole blood/plasma/liquid samples

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