Manual

Product Number: RNK4601

Shipping and Storage

1. All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
2. Inappropriate storage at low temperatures (4℃ or-20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃-25℃).
3. Buffer WS 2/3 may precipitate crystals after using ethanol for a few days, which does not affect its use. Simply do not absorb the crystals and use the supernatant.
4. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

In recent years, extensive research on RNA interference and regulatory small RNAs urgently requires a reagent kit that can effectively extract RNA of around 15-30 nucleotides in size (including SiRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover small molecule RNA, and phenol/guanidine extraction and isopropanol or ethanol precipitation cannot effectively precipitate and recover small molecule RNA. For serum and plasma samples, it is even more difficult to extract due to its own characteristics. This reagent kit uses a unique Buffer LY to rapidly and directly lyse serum plasma RNA enzymes, with strong organic extraction to remove proteins and DNA. RNA, including small molecules, is adsorbed on a special silicon-based membrane in a centrifuge column under high concentration ethanol. Then, through a series of special Buffer WS rapid rinsing centrifugation steps, Buffer WS further removes impurities such as cell metabolites and proteins. Finally, low salt eluent elutes pure RNA from the silicon-based membrane.

Features

1. The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2. There is also no need for steps such as isopropanol or ethanol precipitation that are prone to losing small molecule RNA.
3. The special Buffer LY formula can handle more serum/plasma samples.
4. Multiple column washes ensure high purity and can be used for RNAi, RT-PCR, Northern blot, and various experiments.

Application

Suitable for purifying cell-free total RNA, including miRNA, from animal and human plasma and serum.

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