RNApure RNA Mini Kit
2024-12-27EASYspin Fast Blood RNA Kit
2024-12-27miEASY microRNA Mini Kit
Product Number: RNK0501
Shipping and Storage
- After adding anhydrous ethanol to Buffer WS1 and Buffer WS2/3, they can be stored at room temperature for one month. If they need to be stored for a longer period of time, please store them at 4℃. However, before use, they should be restored to room temperature first
- Buffer WS2/3 may precipitate crystals after using ethanol for a few days, which does not affect its use. Simply do not absorb the crystals and use the supernatant.
- Transportation is carried out at room temperature without affecting the effectiveness of use
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | RNK0501 50Preps |
| Buffer LY/BD | 4°C light avoidance | 50 ml |
| 70% ethanol | RT | 9ml RNase-free H2O |
| Buffer WS1 | RT | 12 ml |
| Buffer WS2/3 | RT | 10 ml |
| RNase free H2O | RT | 10 ml |
| Adsorption column RA and collection tube | RT | 50 |
| MicroRNA adsorption column MA and collection tube | RT | 50 |
Description
In recent years, extensive research on RNA interference and regulatory small RNAs urgently requires a reagent kit that can effectively extract RNA of around 15-30 nucleotides in size (including siRNA and miRNA). However, traditional RNA extraction methods such as silica gel membranes cannot effectively adsorb and recover small molecule RNA, while phenol/guanidine extraction and ethanol precipitation cannot effectively precipitate and recover small molecule RNA. This reagent kit uses a unique Buffer LY/BD, B-mercaptoethanol to rapidly lyse cells and inactivate cell RNA enzymes, with strong organic extraction to remove proteins and DNA. RNA, including small molecules, is adsorbed onto a special silicon-based membrane in a centrifuge column and then subjected to a series of rapid rinsing centrifugation steps. The rinsing solution further removes impurities such as cell metabolites and proteins, and finally, pure RNA is eluted from the silicon-based membrane using a low salt elution solution.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- It also does not require steps such as ethanol precipitation that are prone to losing small RNA molecules.
- Fast and simple, the operation of a single sample can generally be completed within 30 minutes.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.9~2.0 and almost no DNA residue. It can be used for RNAi, RT-PCR, Northern blot, and various experiments.
Application
Suitable for rapid extraction of miRNAs and other small RNAs from various cell tissues
