Manual

Product Number: DRK0301

Shipping and Storage 

1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity. It should be restored to room temperature before use.

2.Storing at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).

3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	50 Preps	100 Preps	200 Preps
Balance Buffer	RT	5ml	10ml	20ml
Buffer HB	RT	20ml	40ml	80ml
Buffer WB	RT	13 ml	25 ml	50 ml
Buffer EB	RT	10 ml	15 ml	15 ml
Adsorption column EC	RT	50	100	200
Collection tube (2ml)	RT	50	100	200

Description

In the presence of high dissociation salts, DNA fragments selectively adsorb onto the silica matrix membrane inside the centrifuge column. Through a series of rapid rinsing centrifugation steps, Buffer WB removes impurities such as primers, nucleotides, proteins, enzymes, etc. Finally, low salt, high pH Buffer EB elutes pure DNA from the silica matrix membrane.

Features

1.The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.

2.Buffer HB, prepared with high-quality guanidine isothiocyanate, does not contain traditional sol solutions of sodium iodide and perchlorate, and does not inhibit downstream reactions such as enzyme digestion and cloning after recovery.

3.By using high concentration buffer HB, equal volume sol can be obtained, reducing operational steps.

4.The unique Buffer HB formula unifies the functions of sol-gel and binding, so a single kit can be used for various situations such as agarose DNA recovery, PCR product cleaning and purification, enzyme digestion product purification and recovery, saving the cost of purchasing multiple kits.

5.Buffer HB is modulated to a yellow color, which facilitates observation of sol effect and monitoring of pH changes to achieve optimal binding effect, greatly improving recovery efficiency.

6.The improved Buffer HB formula greatly enhances buffering capacity and stability, allowing for pH buffering within the optimal binding range even with significant sample changes.

7.Fast and convenient, without the need for toxic reagents such as phenol and chloroform, and without the need for ethanol precipitation.

Application

It is suitable for DNA recovery from agarose gel, purification and recovery of PCR reaction products, purification and recovery of DNA fragments from enzyme digestion products, purification and recovery after probe labeling, DNA sample concentration, etc.

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