Yeast Genomic Medi Kit
2024-12-05Immobilized /Embedded Tissue DNA Kit
2024-12-05Product Number: DNK3001
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures, and can be dissolved again in a water bath at 37 ℃ for a few minutes. After restoring clarity and transparency, it can be cooled to room temperature before use.
- Proteinase K is stored in a ready-to-use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25 ℃ for at least 6 months, at 4 ℃ for 12 months, and at -20 ℃ for 2 years.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.
Components
| Component | Storage | DNK3001 50 Preps |
| Balance Buffer | RT | 5 ml |
| Buffer ML | RT | 20 ml |
| Buffer CB | RT | 20 ml |
| Buffer IR | RT | 25 ml |
| Buffer WB | RT | 13 ml |
| Poly Carrier | –20°C | 200μl |
| Buffer EB | RT | 15 ml |
| Proteinase K(20mg/ml) | 4°C | 1ml |
| Adsorption column AC and collection pipe | RT | 50 |
Note:Buffer WB-Add the specified amount of ethanol according to the instructions before the first use.
Description
This kit uses a specially designed imported DNA adsorption column and a unique buffer system, making it particularly suitable for separating and purifying genomic DNA from oral/pharyngeal swabs.After lysis and digestion of samples from various sources, DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state (especially equipped with Poly Carrier to easily capture trace amounts of nucleic acids from the system). Then, impurities such as salt, cell metabolites, proteins, etc. are removed through a series of rapid rinsing centrifugation steps. Finally, low salt elution buffer is used to elute pure genomic DNA from the silica matrix membrane.The purified DNA is free of impurities and PCR inhibitors, and can be directly used for PCR analysis. Typical yield 0.5μg-3.5μg/swab.
Features
- There is no need to use toxic reagents such as phenol, nor do steps such as ethanol precipitation.
- Saves time, is simple, and a single sample operation can generally be completed within 20 minutes.
- Equipped with a Poly Carrier for fully collecting special trace amounts of DNA.
- Multiple column rinsing ensures high purity, and the extracted DNA has high purity and stable and reliable quality, making it suitable for various routine operations, including PCR, enzyme digestion, sequencing, Southern hybridization, etc.
Application
Suitable for isolating and purifying genomic DNA from oral/pharyngeal swabs.
