Gel Extraction Kit
2024-12-10Mutifunction DNA Recover Kit
2024-12-10PCR Purification Kit
Product Number: DRK0201
Shipping and Storage
1.All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity. It should be restored to room temperature before use.
2.Storage at low temperatures (4℃ or -20℃) can cause solution precipitation and affect the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃-25℃).
3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.
Components
| Component | Storage | DRK020150Preps | DRK0202100 Preps | DRK0203200Preps |
| Balance Buffer | RT | 5ml | 10ml | 20ml |
| Buffer BB | RT | 30ml | 60ml | 100ml |
| Buffer WB | RT | 13ml | 25ml | 50ml |
| Buffer EB | RT | 10ml | 15ml | 20ml |
| Adsorption column EC | RT | 50 | 100 | 200 |
| Collection tube(2ml) | RT | 50 | 100 | 200 |
Description
In the presence of high dissociation salts, DNA fragments selectively adsorb onto the silica matrix membrane inside the centrifuge column. Then, a series of rapid rinsing and centrifugation steps are carried out to remove impurities such as primers, nucleotides, proteins, enzymes, etc. from the protein solution and Buffer WB. Finally, pure DNA is eluted from the silica matrix membrane using a low salt, high pH elution buffer.
Features
1.The silicon matrix membranes inside the centrifugal adsorption column are all made by imported world-renowned companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
2.We used high-quality binding solution, which does not contain traditional binding solutions such as sodium iodide and perchlorate, and does not inhibit downstream reactions such as enzymatic cleavage and cloning after recovery.
3.Buffer BB is modulated with phenol red to a yellow color, which facilitates monitoring of pH changes and achieves optimal binding effect, greatly improving recovery efficiency.
4.It is fast and convenient, and does not require the use of toxic reagents such as phenol and chloroform, nor does it require ethanol precipitation.
Application
Suitable for purification and recovery of PCR reaction products, enzyme cleaved DNA fragments, probe labeling, DNA sample concentration, etc.
