Plant DNA Mini Kit

Product Number: DNK1501

Shipping and Storage 

1.Buffer AP1 and AP3/E may experience precipitation and precipitation at low temperatures. They can be re dissolved in a water bath at 65℃ for a few minutes (AP3 can be heated before adding ethanol, not after adding ethanol). After restoring clarity and transparency, they can be cooled to room temperature before use.

2.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	50 preps	100 Preps	200 Preps
RNase A(10mg/ml)	-20℃	250μl	500μl	1ml
Buffer AP1	RT	20ml	40ml	80ml
Buffer AP2	RT	7ml	13ml	26ml
Buffer AP3/E	RT	15ml	25ml	50ml
Buffer WB	RT	13ml	25ml	50ml
Buffer EB	RT	15ml	15ml	20ml
Adsorption column AC	RT	50	100	200
Collection tube (2ml)	RT	50	100	200

Description

This reagent kit adopts a DNA adsorption column and a novel unique solution system, suitable for rapid and simple extraction of genomic DNA from plant samples containing phenols, polysaccharides, and enzyme inhibitors. The purification of DNA from one or more 100mg fresh or 20mg dry plant samples can be completed within 30 minutes. The extraction process does not require the extraction of toxic organic compounds such as phenols and chloroform, nor does it require time-consuming isopropanol or ethanol precipitation. It can quickly and efficiently remove impurities such as polysaccharides, phenols, and enzyme inhibitors. The purified DNA can be directly used for PCR, enzyme digestion, and hybridization experiments.

Fresh or dry plant tissues (cells) are ground and then lysed by lysate; Proteins, polysaccharides, and cell debris are precipitated and removed; Then, the genomic DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as polysaccharides, polyphenols, cellular metabolites, proteins, etc. are further removed. Finally, the pure genomic DNA is eluted from the silica matrix membrane using low salt Buffer EB.

Features

1.The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.

2.No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.

3.Fast and simple, the operation of a single sample can generally be completed within 1 hour.

4.Several types of polysaccharides, polyphenols, and multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.7-1.9, which can be directly used for PCR, Southern blot, and various enzyme digestion reactions.

Application

Suitable for rapid extraction of plant tissue cells and fungal genomic DNA.

Note

1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000 rpm.

2.Preheat the required water bath to 65℃ for later use before starting the experiment.

3.Buffer AP3/E contains irritating compounds, and latex gloves should be worn during operation to avoid contact with skin, eyes, and clothing. If it gets on the skin or eyes, rinse with plenty of water or physiological saline.

4.The amount of DNA extracted from plant tissue materials from different sources may vary, with a typical yield of 3-25μg from 100mg fresh tissue.

5.The Buffer EB does not contain chelating agent EDTA and does not affect downstream enzyme cleavage, ligation, and other reactions. Water can also be used for elution, but it should be ensured that the pH is greater than 7.5, as low pH can affect elution efficiency. Wash DNA with water and store it at -20℃. If DNA needs to be stored for a long time, it can be eluted with TE buffer (10mM Tris HCl, 1mM EDTA, pH 8.0). However, EDTA may affect downstream enzyme digestion reactions and can be diluted appropriately when used.

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