Immobilized /Embedded Tissue DNA Kit
2024-12-05Urine DNA Kit
2024-12-05Product Number: DNK3401
Shipping and Storage
- Buffer AP1 and AP2 may experience precipitation and precipitation at low temperatures. They can be re dissolved by taking a water bath at 37°C-65°C for a few minutes, without violent shaking to avoid excessive foam formation. After restoring clarity and transparency, cool to room temperature before use.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK3401 50 Preps | DNK3402 100 Preps | DNK3403 200 Preps |
| Buffer AP1 | RT | 20 ml | 40 ml | 80 ml |
| Buffer AP2 | RT | 7 ml | 13 ml | 26 ml |
| Buffer DD | RT | 10 ml | 10 ml | 20 ml |
| RNase A(10mg/ml) | 4°C | 250μl | 500μl | 1ml |
Description
This reagent kit adopts a unique buffer system, which is particularly suitable for extracting genomic DNA from plant dry powder or fresh plant materials. No need for phenol/chloroform extraction, safe and convenient to use, can maximize the removal of impurity proteins and other organic compounds in cells. There is no limit on the starting weight of the sample, and the experimenter can adjust it flexibly according to their own needs. The extracted genomic DNA fragments are large, highly purified, and of stable and reliable quality. The DNA recovered using this kit can be used for various routine operations, including enzyme digestion, PCR, library construction, Southern hybridization, and other experiments.
Features
- No need to use toxic reagents such as phenol and chloroform.
- Fast and simple, the entire operation process can be completed within 1 hour.
- The results are stable and the yield is high (more than twice that of centrifugal column type), with a typical OD260/OD280 ratio of 1.7~1.9 and a length of 50kb-150kb. It can be directly used for PCR, Southern blot, various enzyme digestion reactions, and library construction.
