Marine animal Tussie DNA Kit
2024-12-05Saliva DNA collection And Extraction kit
2024-12-05Plant Max Genomic DNA Kit
Product Number: DNK3801
Shipping and Storage
- When Buffer AP1 and AP3/E are at low temperatures, precipitation and precipitation may occur. They can be dissolved again in a water bath at 65°C for a few minutes (Buffer AP3/E can be heated before adding ethanol, but not after adding ethanol). After restoring clarity and transparency, they can be cooled to room temperature before use.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK3801 10 Preps |
| RNase A(10mg/ml) | -20°C | 500u1 |
| Buffer AP1 | RT | 50ml |
| Buffer AP2 | RT | 20ml |
| Buffer AP3/E | RT | 40ml |
| Buffer WB | RT | 25ml×2 |
| Buffer EB | RT | 20ml |
| Adsorption column AC | RT | 10 |
| Collection tube (50ml) | RT | 10 |
Description
This reagent kit adopts a DNA adsorption column and a novel unique solution system, suitable for rapid and simple extraction of genomic DNA from plant samples containing phenols, polysaccharides, and enzyme inhibitors. The purification of DNA from one or more fresh or 200mg dry plant samples can be completed in about 1 hour. The extraction process does not require the extraction of toxic organic compounds such as phenols and chloroform, nor does it require time-consuming isopropanol or ethanol precipitation. It can quickly and efficiently remove impurities such as polysaccharides, phenols, and alcohol inhibitors. The purified DNA can be directly used for PCR, alcohol cleavage, and hybridization experiments.
Fresh or dry plant tissues (cells) are ground and then lysed in the lysate, and proteins, polysaccharides, and cell debris are precipitated and removed; Then, the genomic DNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as polysaccharides, polyphenols, cellular metabolites, proteins, etc. are further removed. Finally, pure genomic DNA is eluted from the silica matrix membrane in a low salt elution buffer.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of imported specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Fast and simple, the operation of a single sample can generally be completed within 1 hour.
- Several types of polysaccharides, polyphenols, and multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.7~1.9, which can be directly used for PCR, Southern blot, and various alcohol cleavage reactions.
Application
Suitable for rapid extraction of genomic DNA from plant tissues, cells, and fungi.
