Manual

Product Number: RNK3302

Shipping and Storage

1.Therefore, transportation and storage are carried out at room temperature (15℃-25℃). The Buffer RL can be transported at room temperature, and can be stored for a long time in a dark place at 4℃ after receipt. Storage at room temperature for 3 months does not affect the quality of use.

2.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.

3.This reagent kit is stored at room temperature for 12 months without affecting its effectiveness.

Component

Component	RNK3302 50rxns
Buffer RL (4 ℃, dark)	50 ml
Buffer PE	16 mlAdd the specified amount of ethanol according to the label instructions before first use
Buffer RW	10 mlAdd the specified amount of ethanol according to the label instructions before first use
RNase-free H2O	5 ml
RNase free adsorption column RA	50
Collection tube (2ml)	50

Description

The improved guanidine isothiocyanate/phenol one-step method (TRIzol method) cleaves cells and inactivates RNA enzymes. Then, total RNA is selectively adsorbed on the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as cell metabolites and proteins are removed from the membrane using deproteinized and rinsed solutions. Finally, pure RNA is eluted from the silica matrix membrane using low salt RNase free water.

Features

1.The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.

2.Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step reagent, it does not require isopropanol precipitation and ethanol washing process. RNA can be directly eluted from the centrifugation column to avoid the problem of excessive drying and difficult dissolution.

3.Unique Buffer RL formula that effectively eliminates genomic contamination.

4.Multiple rinsing and deproteinization processes result in higher purity of RNA extraction.

5.Effectively removed the content of 5S in total RNA and improved purity.

Note

1.Before the first use, please add the specified amount of ethanol to the Buffer RW bottle and the Buffer PE bottle. After adding, please mark with a tick that ethanol has been added in a timely manner to avoid adding it multiple times!

2.This reagent kit exhibits excellent inhibition of RNA enzymes, and all centrifugation steps can be performed at room temperature unless otherwise specified.

3.The Buffer RL and the Buffer PE contain irritating and harmful compounds. When operating, latex gloves should be worn to avoid contact with skin, eyes, and clothing. If it comes into contact with the skin or eyes, rinse with plenty of water or physiological saline.

4.Considering environmental protection issues, this reagent kit does not contain commonly used laboratory reagent chloroform, and you need to prepare your own chloroform before use. But if chloroform is really difficult to obtain, it can also be avoided.

5.Conventional agarose gel electrophoresis and denaturing gel electrophoresis can be used to analyze the quality of RNA. A good RNA product should show two distinct dominant ribosomal RNA bands after electrophoresis, namely~5Kb (28S) and~2Kb (18S), with a band brightness ratio of approximately 2:1.Sometimes~0.1kb and 0.3Kb (5S, tRNA) bands can also be seen. But sometimes it is normal to see 4-5 bands according to different species, such as certain plant tissues. If the precursor of RNA is immature or uneven nuclear RNA or small nuclear RNA is extracted, discontinuous high molecular weight bands between 7Kb and 15Kb may also be seen.

6.When testing the OD₂₆₀/OD₂₈₀ absorbance ratio, TE (pH 8) should be used to dilute the RNA sample. If diluted with water and tested, due to the low water ion strength and pH value, OD₂₈₀ will increase, resulting in a decrease in the ratio.

7.After adding Buffer RL homogenate and before adding chloroform, the sample can be stored at -60℃ -70℃ for more than one month.

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