RNAsafe RNase inactivator
2024-12-27RNAlong RNA Storage Buffer
2024-12-27Poly Carrier
Product Number: RNK1701
Shipping and Storage
Room temperature for 6 months
Description
Ethanol low-temperature precipitation is the most commonly used method for recovering DNA and RNA from liquid samples. However, ethanol precipitation cannot completely recover the nucleic acid in the sample, at least about 30% of the nucleic acid is lost. If the nucleic acid concentration in the liquid sample is very low or the DNA is less than 200bp, ethanol precipitation can only recover 50% of DNA and RNA. Poly Carrier is a molecular biology grade poly polymer solution. Adding 5-10μl of Poly Carrier during ethanol precipitation can significantly improve the yield of nucleic acid precipitation and achieve a trace DNA recovery rate of 95-98%. At the same time, it can selectively remove short primer (<22bp) fragments and dNTP for precipitation recovery of labeled probes and removal of unlabeled dNTP. Compared with biologically derived nucleic acid precipitation aids such as glycogen and tRNA, Poly Carrier itself has no nucleic acid contamination, no DNase and RNA enzyme activity, and does not affect enzyme digestion, ligation, transcription, PCR, transformation transfection, etc., nor does it affect nucleic acid electrophoresis and DNA protein interaction. Poly Carrier has become the most commonly used nucleic acid precipitant.
Feature
- Significantly increase the yield of DNA or RNA precipitation.
- The recovery of trace amounts of DNA and RNA (20pg) reached 95-98%.
- It does not affect enzyme digestion, ligation, transcription, PCR and other reactions.
- Does not affect electrophoresis and DNA protein interactions.
