Manual

Product Number: QDHC01

Shipping and Storage 

1.Store below 30°C. It is valid for 12 months.

2.Transport at normal temperature, not suggested over 14 days.

3.Opened but not completely used HCMV PCR Master Mix should be stored at (-20±5)°C. It is recommended to separate in PCR tubes before refrigeration to avoid repeated freezing and thawing of all reagents next time. Storage time should not exceed 21 days.

Components

Reagent	Components	QDHC01 48T
HCMV PCR Master Mix	Primers, probes, and reaction buffers,Mg2+,dNTPs,Taq DNA Polymerase	Lyophilized powder
HCMV quantitative standard 1	Recombinant plasmid containing target gene	Lyophilized powder
HCMV quantitative standard 2	Recombinant plasmid containing target gene	Lyophilized powder
HCMV quantitative standard 3	Recombinant plasmid containing target gene	Lyophilized powder
HCMV quantitative standard 4	Recombinant plasmid containing target gene	Lyophilized powder
Positive Control	Recombinant plasmid containing target gene	Lyophilized powder
Negative Control	Physiological saline	1.00mL
Redissolved Diluent	Purified water	1.40mL

Note: 1.For the specific concentrations of HCMV quantitative standard substances 1~4, please refer to the given values in the kit.

2.Do not mix reagents from different batches.

3.Material Required but Not Provided:

3.1Applicable Instrument: ABI7500, ABI 7500 Fast and other Real-time fluorescence PCR instrument with FAM, VIC channels.

3.2Vortex shaker, palm centrifuge, pipette are needed.

Description

This kit utilizes real-time fluorescent PCR detection technology, and uses specific primers and probes for HCMV to achieve quantitative detection of HCMV DNA in serum or plasma samples. The kit is provided with an internal control(IC), which can monitor whether there is PCR inhibitor in the sample to be tested by detecting whether the internal control is normal or not, so as to avoid false negative PCR.

Sample Requirements

1.Applicable sample type: serum or plasma samples.

2.Sample collection:

2.1.Serum: Use a sterile syringe to draw 2mL of the subject's venous blood and inject it into a sterile centrifuge tube. Leave it at room temperature for no more than 4 hours. Centrifuge at 2000rpm for 5 minutes. Take the upper serum (be careful not to bring in red blood cells) and transfer it to another sterile centrifuge tube for use.

2.2.Plasma: 2mL of venous blood is drawn with a disposable sterile syringe and injected into a glass tube containing EDTA anticoagulant. Immediately invert the glass tube slightly and mix it for 5 to 10 times to fully mix the anticoagulant and venous blood. Centrifuge at 1500 rpm for 5 minutes; Suck the upper plasma and transfer it to a 1.5mL centrifuge tube for standby.

3.Sample storage and transportation：

The serum to be tested should be stored at 2~8°C for no more than 72 hours, and at -20°C for no more than 3 months. Samples are shipped in curling or ice-filled foam boxes.

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