Product Number: RA100s
Shipping and Storage
1.The complete reagent kit is stored in a dark place at 2-8℃. After initial use, it is recommended to store RNA HS Reagent at room temperature and avoid light; RNA HS Buffer storage at room temperature; RNA HS Standard 1 and 2 were stored at 2-8℃.
2.Adjust transportation methods according to different destinations.
Components
| Component | RA100s 100preps | RA100s 500preps |
| RNA HS Reagent (200 × in DMSO) | 250µl | 1.25ml |
| RNA HS Buffer | 50ml | 250ml |
| RNA HS Standard 1 (0 ng/µl in TE buffer) | 1ml | 5ml |
| RNA HS Standard 2 (10 ng/µl in TE buffer) | 4×250µl | 10×500µl |
Description
RNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This reagent kit includes fluorescence detection reagents, buffer solutions, and RNA standards. This reagent kit has high selectivity for RNA, is not affected by dsDNA, and has excellent linear relationships with RNA samples in the 5-100 ng range. It can accurately quantify total RNA, rRNA, and mRNA samples with concentrations ranging from 250pg/µ l to 100ng/µ l, and has excellent tolerance to some conventional pollutants such as salt, free nucleotides, proteins, solvents, and detergents. This product is easy to operate and can be performed at room temperature. Before use, dilute the fluorescence detection reagent with buffer to a working solution, then add the RNA sample to be tested and detect it using a Qubit fluorescence meter.
Application
Total RNA、rRNA、mRNA from 250 pg/μl to 100ng/μl
Note
1.Fluorescent dyes have quenching issues, please try to avoid light as much as possible.
2.For detection reagents and RNA standards, mix them upside down before each use, and briefly centrifuge for 1-2 seconds to collect the reagents at the bottom of the tube.
3.To avoid degradation of RNA standards, please use RNA free consumables for the experiment and store the standards at 2-8℃ after the experiment is completed.
4.To ensure the accuracy of quantitative results, please use a calibrated pipette for operation.
5.Please perform quantitative testing at room temperature. Before use, place the components in the reagent kit at room temperature. During the experiment, please do not hold the PCR tube with your hands for a long time.
6.Please make sure to complete the testing of all samples within 3 hours of preparing the working solution to avoid deviation in the results caused by fluorescence quenching.
