RNA HS Assay kit

Product Number: RA100s

Shipping and Storage 

1.The complete reagent kit is stored in a dark place at 2-8℃. After initial use, it is recommended to store RNA HS Reagent at room temperature and avoid light; RNA HS Buffer storage at room temperature; RNA HS Standard 1 and 2 were stored at 2-8℃.

2.Adjust transportation methods according to different destinations.

Components

Component	RA100s 100preps	RA100s 500preps
RNA HS Reagent (200 × in DMSO)	250µl	1.25ml
RNA HS Buffer	50ml	250ml
RNA HS Standard 1 (0 ng/µl in TE buffer)	1ml	5ml
RNA HS Standard 2 (10 ng/µl in TE buffer)	4×250µl	10×500µl

Description

RNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This reagent kit includes fluorescence detection reagents, buffer solutions, and RNA standards. This reagent kit has high selectivity for RNA, is not affected by dsDNA, and has excellent linear relationships with RNA samples in the 5-100 ng range. It can accurately quantify total RNA, rRNA, and mRNA samples with concentrations ranging from 250pg/µ l to 100ng/µ l, and has excellent tolerance to some conventional pollutants such as salt, free nucleotides, proteins, solvents, and detergents. This product is easy to operate and can be performed at room temperature. Before use, dilute the fluorescence detection reagent with buffer to a working solution, then add the RNA sample to be tested and detect it using a Qubit fluorescence meter.

Application

Total RNA、rRNA、mRNA from 250 pg/μl to 100ng/μl

Note

1.Fluorescent dyes have quenching issues, please try to avoid light as much as possible.

2.For detection reagents and RNA standards, mix them upside down before each use, and briefly centrifuge for 1-2 seconds to collect the reagents at the bottom of the tube.

3.To avoid degradation of RNA standards, please use RNA free consumables for the experiment and store the standards at 2-8℃ after the experiment is completed.

4.To ensure the accuracy of quantitative results, please use a calibrated pipette for operation.

5.Please perform quantitative testing at room temperature. Before use, place the components in the reagent kit at room temperature. During the experiment, please do not hold the PCR tube with your hands for a long time.

6.Please make sure to complete the testing of all samples within 3 hours of preparing the working solution to avoid deviation in the results caused by fluorescence quenching.

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