EASYspin Yeast RNA Fast Kit
2024-12-27RNA keeper Stabilization Buffer
2024-12-27Product Number: RNK1402
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Component
| Component | Storage | RNK1402 50preps |
| Buffer RC | RT | 20 ml |
| Buffer RW | RT | 10 ml |
| RNase-free H2O | RT | 10 ml |
| RNase free adsorption column RA | RT | 50个 |
| Collection tube (2ml) | RT | 50个 |
Description
This reagent kit uses centrifugal adsorption columns and silicon matrix membranes, all of which are specially imported adsorption membranes. The difference in adsorption capacity between columns is minimal, and the repeatability is good. Under high salt conditions, RNA binds efficiently and specifically to the silica gel adsorption membrane, while maximizing the removal of proteins, inorganic salt ions, and many organic impurities. Under low salt conditions, RNA is eluted. The amount of RNA samples that can be processed can reach up to 50μg. This kit is used for purifying and recovering RNA from enzyme reaction solutions (such as DNase treatment, protease treatment, RNA labeling, etc.), and can also be used for purifying RNA extracted from other methods. The purified total RNA is free of protein contamination, and the obtained RNA can be used for Northern blot, Dot blot, mRNA extraction, cDNA synthesis, primer extension, differential display, etc.
