Manual

Product Number: RNK2302

Shipping and Storage

1. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃). Buffer RLS can be transported at room temperature, and can be stored for a long time in a dark place at 4℃ after receipt. Storage at room temperature for 3 months does not affect the quality of use.
2. To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Description

The improved one-step method of guanidine isothiocyanate/phenol (TRIzol LS method for liquid samples) lyses cells and inactivates RNA enzymes. Then, total RNA is selectively adsorbed onto the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, Buffer RE and Buffer RW remove impurities such as cell metabolites and proteins, and finally, low salt RNase free water elutes pure RNA from the silica matrix membrane.

Features

1. The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.
2. Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step reagent, RNA can be directly eluted from the centrifugation column without the need for isopropanol precipitation and ethanol washing process, avoiding the problem of excessive drying and difficult dissolution.
3. The unique Buffer RLS formula can directly lyse whole blood without the need to first lyse and remove red blood cells.
4. Multiple rinses to remove proteins result in higher purity of extracted RNA.
5. Effectively removed the content of 5S in total RNA and improved purity.

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