Manual

Product Number: RNK0302

Shipping and Storage

1.Therefore, transportation and storage are carried out at room temperature (15℃-25℃). The Buffer RL can be transported at room temperature, and can be stored for a long time in a dark place at 4℃ after receipt. Storage at room temperature for 3 months does not affect the quality of use.

2.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly after use.

3.This reagent kit is stored at room temperature for 12 months without affecting its effectiveness.

Component

Description

The improved guanidine isothiocyanate/phenol one-step method (TRIzol method) cleaves cells and inactivates RNA enzymes. Then, total RNA is selectively adsorbed on the silica matrix membrane in a highly dissociated salt state. Through a series of rapid rinsing centrifugation steps, impurities such as cell metabolites and proteins are removed from the membrane using deproteinized and rinsed solutions. Finally, pure RNA is eluted from the silica matrix membrane using low salt RNase free water.

Features

1.The silicon matrix membranes inside the centrifugal adsorption column are all made of specially designed adsorption membranes, with minimal differences in adsorption capacity between columns and good repeatability.

2.Combining the advantages of good stability, high purity, and convenient and fast centrifugation column of guanidine isothiocyanate/phenol one-step reagent, it does not require isopropanol precipitation and ethanol washing process. RNA can be directly eluted from the centrifugation column to avoid the problem of excessive drying and difficult dissolution.

3.Unique Buffer RL formula that effectively eliminates genomic contamination.

4.Multiple rinsing and deproteinization processes result in higher purity of RNA extraction.

5.Effectively removed the content of 5S in total RNA and improved purity.

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