eECL Western Blot Kit
2023-10-22
ARCA Ammonium Solution
2023-11-28RNase inhibitor, Murine
Product Number: RNK3501
Shipping and Storage
Store at -20°C.
Components
| Component | RNK3501 30μL | RNK3501 250μL | RNK3501 1mL |
| RNase inhibitor, Murine (40 U/μL) | 30μL | 250μL | 1mL |
Description
It is a spectrum RNase inhibitor with a molecular weight of approximately 50KDa. It can specifically bind to RNase by non-covalent bond to form a complex to inactivate RNase without inhibiting activities of RNase H, nuclease S1, SP6, T7 or T3 RNA polymerase, AMV or M-MLV reverse transcriptase, Taq DNA polymerase and RNase T1. RNasin does not affect subsequent reverse transcription and translation processes. It is widely used in RNA research such as RT-PCR, cDNA synthesis, mRNA protection, in vitro transcription and in vitro translation, preparation of RNase-Free antibodies, in situ hybridization and mRNA localization.
Application
- cDNA synthesis.
- In vitro translation.
- In vitro transcription.
- RNA amplification.
- RNA purification and storage
Unit definition
One unit is defined as the amount of RNasin required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2´,3´-cyclic monophosphate by ribonuclease A.
Storage Buffer
20 mM HEPES-KOH (pH7.6), 50 mM KCl, 8 mM DTT, 50% (v/v) Glycerol.
Purity
- 300U RNase inhibitor, Murine, and 1μg λDNA-Hind III decomposition product reacted at 37℃ for 1 hour without any change in the electrophoresis band of DNA.
- 300U RNase inhibitor, Murine, and 1μg Superhelix pBR322 DNA reacted at 37℃ for 1 hour without any change in the electrophoresis band of DNA
- 300U RNase inhibitor, Murine, and 1μg 16S,23S rRNA reacted at 37℃ for 1 hour without any change in the electrophoresis band of RNA
Note
- This product should avoid repeated freezing and thawing. Please store it at -70 ℃ for long-term storage.
- Suggest using a final concentration of 1U/ μL.
