Product Number: RNK5001
Shipping and Storage
Room temperature (15-30℃).
Components
| Component | Storage | RNK5001 50preps |
| Buffer LY | 4°C | 40ml |
| Buffer SA | RT | 5ml |
| Buffer RW1 | RT | 40ml |
| Buffer RW | RT | 10ml |
| RNase-free H2O | RT | 10ml |
| DNase Buffer | -20°C | 1.5ml ×2 |
| RNase free DNase I | -20°C | 250μl |
| RNA fixer(RNA later) solution | RT | 70ml |
| RNA Binding Columns | RT | 50 |
Note: All reagents, when store in indicated temperature, are stable for 9 months.
Description
RNAstore Blood RNA Kit is designed for isolation of total RNA from blood samples stored in RNA fixer(Same as RNA later) Solution. Blood samples can be processed immediately, or they can be stored in RNA fixer Solution(provided with the kit) for a few days at ambient temperature or for prolonged periods at -20℃ prior to RNA extraction. The Kit RNA isolation procedure consists of two parts:
- Cell lysis in a guanidinium-based solution and initial purification of the RNA by phenol/chloroform extraction
- Final RNA purification by solid-phase extraction on a glass fiber filter. This procedure completely removes contaminants and enzyme inhibitors producing high-quality RNA. RNA purified using the Kit is ready for applications such as qRT-PCR.
Materials and Equipment to be Supplied by User
- Microcentrifuge capable of 13,000×g
- 100% ethanol, water-saturated-Phenol: Chloroform( 5:1)
- RNase-free filter pipette tips
- Blood collection tubes (recommended anticoagulant: potassium EDTA or sodium EDTA)
- 1.5 or 2.0ml microcentrifuge tubes
- Shaking incubators or heat blocks capable of 75°C-90°C
