Soil DNA Fast Kit

Product Number: DNK2701

Shipping and Storage 

1.Buffer LYS or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37 ℃ for a few minutes. After restoring clarity and transparency, it can be used by cooling to room temperature. Be careful not to shake vigorously to avoid the generation of a large number of bubbles.

2.Proteinase K is stored in a ready to use glycerol Buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25 ℃ for at least 6 months, at 4 ℃ for 12 months, and at -20 ℃ for 2 years.

3.To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.

Components

Component	Storage	DNK2701 50 Preps
Buffer SUS	RT	25 ml
Buffer LYS	RT	6 ml
Buffer S1	RT	15 ml
Buffer S2	RT	15 ml
Buffer S3	RT	30 ml
Buffer IR	RT	25 ml
Buffer WB	RT	13 ml
Buffer EB	RT	10 ml
Proteinase K	4℃	1 ml
Adsorption column AC and collection tube	RT	50

Description

The soil genomic DNA extracted by ordinary handheld or reagent kits often fails due to the presence of strong inhibitors of PCR, such as humic acid and palmitic acid impurities. In addition, the use of severe glass bead impacts to rupture bacterial cells often leads to DNA cleavage and degradation. Our company has developed soil genomic DNA with independent intellectual property rights through long-term research and development. By using patented humic acid and palmitic acid removal reagents combined with specially treated purification columns, these impurities can be maximally removed. At the same time, multiple column washes are added to ensure that the obtained DNA has extremely high purity. In addition, the unique extraction and lysis system can quickly lyse cells (walls) and inactivate intracellular nucleases, It does not require the use of glass beads to break walls, effectively ensuring the integrity of genomic DNA.

Features

1.Our company's unique patented formula and purification column can effectively remove impurities such as humic acid.

2.Without the need for glass bead breaking, the integrity of genomic DNA is effectively ensured, with a length of up to 30kb-50kb. It can be directly used for PCR, Southern blot, and various enzyme digestion reactions.

3.Strong compatibility, suitable for various types of soils, including difficult to extract soils such as silt.

4.Multi step removal of various impurities and inhibitors ensures extremely high purity, with a typical OD260/OD280 ratio of 1.7-1.9.

5.No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.

6.Fast and simple, the operation of a single sample can generally be completed within 60 minutes.

Application

Suitable for rapid extraction of various soil genomic DNA

Note

1.All centrifugation steps are completed at room temperature using a traditional desktop centrifuge with a speed of up to 13000rpm.

2.Preheat the water bath to 37 ℃ or 70 ℃ as needed before starting the experiment.

3.Buffer S3 and Buffer IR contain irritating compounds. When operating, wear latex gloves to avoid contact with skin, eyes, and clothing. If it gets on the skin or eyes, rinse with plenty of water or physiological saline.

4.Buffer EB does not contain chelating agent EDTA and does not affect downstream enzyme cleavage, linking, and other reactions. Water can also be used for elution, but it should be ensured that the pH is greater than 7.5, as low pH can affect elution efficiency. Wash DNA with water and store it at -20 ℃. If DNA needs to be stored for a long time, it can be eluted with TE Buffer (10mM Tris HCl, 1mM EDTA, pH 8.0). However, EDTA may affect downstream enzyme digestion reactions and can be diluted appropriately when used.

---