Blood and Tissue DNA Mini Kit,50 preps
2024-12-02Genomic Medium Kit
2024-12-02Product Number: DNK0801
Shipping and Storage
- When the ambient temperature is low, some detergent ingredients in Buffer NLY will precipitate and become turbid or precipitate. It can be heated in a 37℃ water bath for a few minutes to restore clarity. Do not shake violently to avoid excessive foam formation.
- Buffer PP may experience precipitation and precipitation, and can be re dissolved by taking a water bath at 37℃ for a few minutes. If it cannot be completely dissolved, it will not affect the effectiveness of use. Simply take the upper solution.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK0801 50 Preps | DNK0802 100 Preps | DNK0803 200 Preps |
| Buffer NLY | RT | 30 ml | 60 ml | 120 ml |
| Buffer PP | RT | 10 ml | 20 ml | 40 ml |
| Buffer DA | RT | 10 ml | 15 ml | 30 ml |
| RNase A(10mg/ml) | -20℃ | 100 µl | 200 µl | 400 µl |
Description
This kit is used for rapid extraction of genomic DNA from plant cells/tissues. After grinding or homogenizing the sample, buffer NLY is added. Firstly, cells are lysed under strong detergents or in synergy with Proteinase K to release genomic DNA. Then, RNase A is added to remove RNA, followed by selective precipitation of buffer PP to remove protein. Finally, pure genomic DNA is precipitated in isopropanol and re dissolved in buffer DA.
Features
- No need to use toxic reagents such as phenol and chloroform.
- Fast and simple, the entire process of organizing sample operations can be completed within 1 hour.
- The results are stable and the yield is high (more than twice that of centrifugal column type), with a typical OD260/OD280 ratio of 1.7~1.9 and a length of 50kb-150kb. It can be directly used for PCR, Southern blot, various enzyme digestion reactions, and library construction.
