Manual

Product Number: RNK0201

Shipping and Storage

Trizoe LS Reagent can be stably stored at room temperature for 12 months. However, for optimal results, we recommend storing in an environment of 2-8°C.  

Important Note

Toxic substances that come into contact with the skin or are accidentally swallowed can cause burns. Once in contact with the skin, wash immediately with a large amount of detergent and clear water. If you feel unwell, see a doctor and seek the correct treatment plan for phenol and other components.

Description

The Trizoe LS Reagent reagent is a reagent that extracts total RNA directly from liquid samples derived from humans, animals, plants, yeast, bacteria, and viruses. It can maintain the integrity of RNA during cell fragmentation and lysis. After adding chloroform and centrifuging, the sample is divided into a water sample layer and an organic layer. RNA exists in the water sample layer. After collecting the water sample layer above, RNA can be reduced through isopropanol precipitation. After removing the water sample layer, the DNA and proteins in the sample can also be successively reduced by precipitation. Ethanol precipitation can precipitate DNA from the intermediate layer, while adding isopropanol to the organic layer can precipitate protein. Co purified DNA is very useful for standardizing RNA production between samples.

This method has good separation effects on small amounts of tissue (50-100mg) and cells (5×10⁶), as well as large amounts of tissue (≥lg) and cells (>10⁷), whether from human, animal, plant, or bacterial tissues. The simplicity of Trizoe LS Reagent reagent operation allows for simultaneous processing of multiple samples. All operations can be completed within one hour. The total RNA extracted by Trizoe LS Reagent can avoid contamination of DNA and proteins. Therefore, it can perform RNA blotting analysis, dot hybridization, poly (A)+selection, in vitro translation, RNA enzyme protection analysis, and molecular cloning. If used for PCR, it is recommended to use amplification grade DNase I to process the extracted total RNA when two primers are located within a single exon.

The Trizoe LS Reagent reagent can promote the precipitation of various RNAs of different species and molecular weight sizes. For example, RNA agarose gel electrophoresis extracted from rat liver and stained with ethidium bromide showed many discontinuous high molecular weight bands between 7kb and 15kb, two dominant ribosomes~5kb (28S) and~2kb (18S), and low molecular weight RNA between 0.1 and 0.3kb (tRNA, 5S). When the extracted RNA is diluted with TE, its A260/A280 ratio is ≥ 1.8.

Application

Suitable for rapid extraction of total RNA, DNA, and proteins from liquid or solid samples from various sources

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