BAC/PAC Large Scale Plasmid Extraction Kit
2024-12-17Trizoe LS Reagent
2024-12-23Product Number: RNK0101
Shipping and Storage
Trizoe can be stably stored at room temperature for 12 months. Nevertheless, for optimal results, we recommend storing in an environment of 2-8°C.
Important Note
This product contains phenol, which is toxic and corrosive. If inhaled, in contact with the skin, swallowed, etc., it can cause poisoning, burns, and other bodily injuries. When using this product, protective equipment such as protective clothing, gloves, eye masks, face shields, etc. should be worn. If accidentally touched, immediately rinse with plenty of water and seek medical treatment.
Description
Trizoe Reagent is a broad-spectrum total RNA extraction reagent. The experimental operation is fast and convenient, with bright colors and easy layering. This reagent has a wide range of applications and can extract total RNA from animal tissues, plant materials, various microorganisms, and cultured cells. This method has good separation effects on small amounts of tissues (50-100mg) and cells (5×106), as well as large amounts of tissues (≥ 1g) and cells (>107). The sample can be fully lysed in TRIpure while maximizing the integrity of RNA. After centrifugation with chloroform, the solution will be divided into three layers: the upper colorless aqueous phase, the middle layer, and the lower red organic phase, with RNA distributed in the supernatant layer. After collecting the supernatant, total RNA can be recovered by precipitation with isopropanol. The extracted total RNA has good integrity, no protein or DNA contamination, and can be used for various routine molecular biology experiments, such as RT-PCR Real-time RT-PCR、Northern blot、Dot Blot、 External translation, etc.
Trizoe reagent can promote the precipitation of various RNAs of different species and molecular weights. For example, RNA agarose gel electrophoresis extracted from rat liver and stained with ethidium bromide showed many discontinuous high molecular weight bands (mRNA and hnRNA components) between 7 kb and 15 kb, two dominant ribosomes~5 kb (28S) and~2 kb (18S), and low molecular weight RNA between 0.1 and 0.3 kb (tRNA, 5S). When the extracted RNA is diluted with TE, its A260/A280 ratio is ≥ 1.8. Note that for ordinary agarose gel electrophoresis, the position of 28S is about 2kb, and 18S is about 1kb. The position of gel with different concentrations varies greatly.
