Quick PAGE Extraction Kit
2024-12-10High Pure Plasmid Mini Kit
2024-12-16Universal DNA Purification/Concentrate Micro Kit
Product Number: DRK0601
Shipping and Storage
- All solutions should be clear. If the ambient temperature is low, the solution may form precipitates and should not be used directly. It can be heated in a 37℃ water bath for a few minutes to restore clarity. It should be restored to room temperature before use.
- Storing at low temperatures (4℃ or -20℃) can cause solution precipitation, affecting the effectiveness of use. Therefore, transportation and storage are carried out at room temperature (15℃ -25℃).
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DRK0601 100rxns | DRK0602 200rxns |
| Balance Buffer | RT | 5ml | 10ml |
| Buffer DB | RT | 50ml | 100ml |
| Buffer WB | RT | 13 ml | 25ml |
| Add the specified amount of ethanol according to the bottle label instructions before the first use. | |||
| Buffer EB | RT | 10 ml | 20ml |
| Ultratrace DNA centrifuge column | RT | 100 | 200 |
| Collection tube (2ml) | RT | 100 | 200 |
Note: Storing this reagent kit at room temperature for 12 months does not affect its effectiveness.
Description
This product is a specialized reagent kit for ultra trace DNA concentration and recovery. It is applicable to the recovery of agarose gel DNA of trace DNA, purification and recovery of PCR reaction products, purification and recovery of DNA fragments of enzyme digestion products, purification and recovery after probe labeling, DNA sample concentration, etc.
In the presence of high dissociation salts, DNA fragments selectively adsorb onto the silica matrix membrane inside the centrifuge column. Then, through a series of rapid rinsing centrifugation steps, Buffer WB removes impurities such as primers, nucleotides, proteins, enzymes, etc. Finally, pure DNA is eluted from the silica matrix membrane using a low salt, high pH elution buffer.
Features
- The special washer free centrifugal column design ensures no liquid residue and contamination after centrifugation. Ensuring high purity of recycled DNA.
- The special design of a 5ug centrifuge column for trace amounts can achieve a minimum elution of 5ul, ensuring a high concentration of recovered DNA.
- High quality Buffer DB was used, which does not contain traditional sol solutions of sodium iodide and perchlorate, and does not inhibit downstream reactions such as enzyme digestion and cloning after recovery.
- The unique Buffer DB formula unifies the functions of sol-gel and binding, so a single kit can be used for various situations such as agarose DNA recovery, PCR product cleaning and purification, enzyme digestion product purification and recovery, saving the cost of purchasing multiple kits.
- The Buffer DB has been modulated to a yellow color, making it easy to observe the sol effect and monitor pH changes to achieve the best binding effect, greatly improving the recovery efficiency.
- The improved Buffer DB formula greatly enhances buffering capacity and stability, allowing for pH buffering within the optimal binding range even with significant sample changes.
- Fast and convenient, without the need for toxic reagents such as phenol and chloroform, and without the need for ethanol precipitation.
