Cytomegalovirus(CMV) qPCR detection kit
2025-12-16
Food-grade Lipase
2025-12-23Yeast Genomic Fast Kit
Product Number: DNK2001
Shipping and Storage
- Precipitation may occur in Binding Buffer CB or Protein Removal Buffer PE at low temperatures. Incubating in a 37°C water bath for several minutes can help redissolve the precipitates. Use after the solution becomes clear and transparent and cools to room temperature.
- Proteinase K is supplied in a ready-to-use glycerol buffer and shipped at ambient temperature. Upon receipt, it can be stored for at least 6 months at ≤ 25°C, 12 months at 4°C, or 2 years at -20°C.
- Lytic Enzyme is a snail enzyme glycerol stock solution and is therefore viscous. Handle with care. Store at -20°C. Snail enzyme is a mixture of enzymes prepared from the crop and digestive tract of snails, containing over 20 enzymes including cellulase, pectinase, amylase, protease, etc. It is suitable for breaking down the cell walls of various yeasts.
- To prevent volatilization, oxidation, or pH changes due to prolonged exposure to air, cap all solutions tightly immediately after use.
Components
| Component | Storage | DNK2001 50 preps |
| Balance Buffer | RT | 5mL |
| Buffer SE | RT | 15mL |
| Buffer YB | RT | 10mL |
| Buffer CB | RT | 11mL |
| Buffer PE | RT | 16mL |
| Buffer WB | RT | 13mL |
| Buffer EB | RT | 15mL |
| Lytic Enzyme | -20℃ | 2.5mL |
| Proteinase K | 4℃ | 1mL |
| Adsorption column AC | RT | 50 |
| Collection tube (2ml) | RT | 50 |
Description
This kit utilizes centrifugal adsorption columns specifically designed for DNA binding and a unique buffer system for the extraction of genomic DNA from yeast cells. It eliminates the need for toxic reagents like phenol-chloroform and time-consuming alcohol precipitation steps, effectively removing proteins and other inhibitory impurities. The extracted DNA is ready for downstream applications such as restriction enzyme digestion, PCR, and Southern blotting.
Features
- The silica membrane in the centrifugal adsorption columns is made from specially formulated adsorption material, ensuring minimal column-to-column variation in binding capacity and excellent reproducibility. This overcomes the instability issues often associated with membranes in domestic kits.
- No need for toxic reagents like phenol or steps such as ethanol precipitation.
- Fast and simple procedure. Processing after cell lysis can typically be completed within 30 minutes per sample.
- Multiple washing steps ensure high purity, with typical OD260/OD280 ratios ranging from 1.7 to 1.9. The DNA is suitable for direct use in PCR, Southern blotting, and various enzymatic digestions.
Application
Suitable for yeast genomic DNA extraction.
