Apoptotic body/hoeschst Stain Kit
2024-12-05M13 Phage Single Stranded Kit
2024-12-05Product Number: DNK2001
Shipping and Storage
- Buffer CB or Buffer IR may precipitate and precipitate at low temperatures. It can be dissolved again in a water bath at 37℃ for a few minutes to restore clarity and transparency. After cooling to room temperature, it can be used.
- Proteinase K is stored in a ready to use glycerol buffer and transported at room temperature. After receipt, store at room temperature not exceeding 25℃ for at least 6 months, at 4℃ for 12 months, and at -20℃ for 2 years.
- Lytic Enzyme is a snail enzyme glycerol storage solution, which is relatively viscous. Please use it carefully and store at -20℃. Snail enzyme is a mixed enzyme prepared from the snail's pouch and digestive tract. It contains more than 20 enzymes, including cellulase, pectinase, amylase, protease, etc. Suitable for breaking and dissolving various yeast cell walls.
- To avoid volatilization, oxidation, and pH changes caused by prolonged exposure of reagents to the air, each solution should be covered tightly in a timely manner after use.
Components
| Component | Storage | DNK2001 50 preps |
| Balance Buffer | RT | 5 ml |
| Buffer YB | RT | 20 ml |
| Buffer CB | RT | 11 ml |
| Buffer IR | RT | 25 ml |
| Buffer WB | RT | 13 ml |
| Buffer EB | RT | 15 ml |
| Lytic Enzyme | -20℃ | 2.5 ml |
| Proteinase K | 4℃ | 1 ml |
| Adsorption column AC | RT | 50 |
| Collection tube (2ml) | RT | 50 |
Description
This kit uses a DNA adsorption column and a unique solution system, suitable for rapid and simple extraction of genomic DNA from yeast cultures from various sources. Approximately 3ml of yeast culture medium in exponential growth stage can generally purify 10-15μg of high-quality genomic DNA in one extraction. The purified DNA product can be directly used for experiments such as PCR, enzyme digestion, and hybridization. After yeast cells are treated with Lytic Enzyme to remove the cell wall, the unique Buffer CB/Proteinase K rapidly lyses the cells and inactivates intracellular nucleases. Then, genomic DNA selectively adsorbs onto the silica matrix membrane in a high dissociation salt state. Through a series of rapid rinsing centrifugation steps, Buffer IR and Buffer WB remove impurities such as cell metabolites and proteins, Finally, low salt Buffer EB elutes pure genomic DNA from the silica matrix membrane.
Features
- The silicon matrix membrane inside the centrifugal adsorption column is entirely made of specially designed adsorption membranes from world-renowned imported companies, with minimal differences in adsorption capacity between columns and good repeatability. Overcoming the drawback of unstable membrane quality in domestic reagent kits.
- No toxic reagents such as phenol are required, and no steps such as ethanol precipitation are required.
- Fast and simple, the operation after cracking a single sample can generally be completed within 30 minutes.
- Multiple column washes ensure high purity, with a typical OD260/OD280 ratio of 1.7-1.9, which can be directly used for PCR, Southern blot, and various enzyme digestion reactions.
Application
Suitable for rapid extraction of various yeast genomic DNA
