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2025-02-1915 Types of Hemorrhagic Fever Pathogens Multiplex Nucleic Acid Detection Kit (Real-Time PCR Method)
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Product Number: DTK534
Shipping and Storage
- The reagent kit should be stored in a cold and dark place below -15 ℃; Valid for 12 months; Sealed transportation with foam box and ice bag, the temperature shall not exceed 8 ℃; The production date and expiration date are detailed in the outer packaging box.
- The reagent kit should avoid repeated freeze-thaw cycles, with no more than 2 freeze-thaw cycles.
- After opening, store in a cold and dark place below -15 ℃ without affecting the use within the expiration date.
Component
| Component | 24T |
| 15 types of hemorrhagic fever pathogen RT-PCR reaction solution | 24×8-tube in bag A and 24×8-tube in bag B (take one 8-tube each from bag A and bag B to make 1T) |
| Positive controls for 15 hemorrhagic fever pathogens (universal) | 240μL×2 |
| 15 types of hemorrhagic fever pathogen negative controls (universal) | 480μL |
Required but not provided: Nucleic acid extraction or purification kits produced by our company
Description
This reagent kit utilizes real-time fluorescence PCR principle to qualitatively detect specific genes of 15 hemorrhagic fever pathogens.
This kit uses real-time fluorescence PCR technology, and each tube of reaction solution contains specific amplification primers and detection probes for each target gene. The probes are oligonucleotide sequences containing 5 'labeled luminescent groups and 3' labeled quenching groups. If the probes remain intact, the fluorescence signal emitted by the luminescent groups will be absorbed by the quenching groups and no signal will be detected. During the PCR amplification process, probes that specifically bind to the template are cleaved by Taq enzyme (with 5 '-3' exonuclease activity), and the luminescent and quenching groups are separated, producing a fluorescent signal. Real time monitoring and output of signal strength of corresponding channels during PCR process using instruments to achieve qualitative analysis of detection results.
Application
This kit is suitable for qualitative detection of 15 hemorrhagic fever pathogens in blood samples, cerebrospinal fluid, tissue specimens (including brain tissue, liver, and spleen), and mosquito specimens. The test results are for scientific research purposes only and cannot be used alone as a basis for clinical diagnosis or exclusion of cases.
The main pathogens detected are as follows: chikungunya virus, yellow fever virus, dengue virus types I, II, III, IV, Hantaan virus, Seoul virus, Pumala virus, Rift Valley fever virus, Congo Crimean hemorrhagic fever virus, Marburg virus, Ebola virus, Lassa fever virus, and New Bunyavirus, which are 15 common hemorrhagic fever pathogens.
