Manual

Product Number: DTK183

Shipping and Storage

1. -20 ℃± 5 ℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
2. The collected or processed samples should be stored at 2 ℃~8 ℃ for no more than 24 hours; If long-term storage is required, it should be stored at -70 ℃ or below, with no more than 3 freeze-thaw cycles.

Component

Note: Different batches of reagents cannot be mixed.

Description

This kit uses TaqMan probe method for real-time fluorescence PCR technology, designs a pair of bluetongue virus specific primers, combines with a specific probe, and uses fluorescence PCR technology to amplify and detect the RNA of bluetongue virus in vitro, which is used for pathogen diagnosis of suspected infected individuals in clinical practice.

Application

Bluetongue disease is a non-contact infectious disease mainly affecting ruminant animals such as sheep or cattle caused by the bluetongue virus (BTV) of the family Enterotoxidae. It is mainly transmitted by bites from midges on their ruminant hosts. Sheep, goats, and cattle are the main susceptible animals to this disease, while camels and wild ruminant animals can all be infected. Its symptoms are severe catarrhal inflammation of the cheek mucosa and gastrointestinal mucosa. Infected animals are mentally depressed, lose appetite, often experience bloody diarrhea, and often limp due to the shedding of the hoof crest epithelium. At present, there is no highly effective prevention and treatment method for bluetongue disease. The rapid diagnosis and treatment of the pathogen of bluetongue disease are the main methods for controlling this disease.

This kit is suitable for detecting bluetongue virus RNA in animal spleen, lymph node, liver, kidney tissue, semen samples and other specimens, and is suitable for auxiliary diagnosis of bluetongue virus infection.

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