Paenibacillus mucilaginosus Probe-Based qPCR Kit
2025-03-19Bacillus cereus (BC) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-03-19Product Number: DTK144
Shipping and Storage
- -20℃±5℃, stored in the dark, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a validity period of 12 months.
- The collected or processed samples should be stored at 2℃~8℃ for no more than 24 hours; If long-term storage is required, it should be stored at -70℃ or below, with no more than 3 freeze-thaw cycles.
Component
| Component | 50T |
| BP reaction solution | 500μL×2 |
| Enzyme solution | 50μL |
| BP positive control product | 250μL |
| Negative quality control product | 250μL |
Note: Different batches of reagents cannot be mixed.
Description
This kit uses TaqMan probe method for real-time fluorescence PCR technology, designs a pair of Burkholderia mallei specific primers, and combines them with a specific probe to perform in vitro amplification and detection of Burkholderia mallei DNA using fluorescence PCR technology, which is used for pathogenic diagnosis of suspected infectious materials in clinical practice.
Application
Melioidosis is a tropical zoonotic disease caused by Burkholderia pseudomallei (BP) infection. Infection occurs through mucosal contact, inhalation, and ingestion of BP. Clinical manifestations include multiple abscesses, refractory pneumonia, and fatal sepsis, with a mortality rate of 20-60%. Melioidosis is commonly found in Southeast Asia, with cases mainly concentrated in Vietnam, Myanmar, and Malaysia. The northern part of Australia is also a popular area. The epidemic areas of glanders in China are mainly concentrated in Hainan, Guangdong and other places. Sheep, goats, horses, pigs, cows, dogs, cats and other animals also carry glanders and can transmit similar diseases to humans. Wounds or abrasions on human skin may become infected if they come into contact with contaminated soil or water. People may also be infected by inhaling contaminated soil dust and swallowing or inhaling contaminated water.
This kit is suitable for detecting clinical specimens suspected of Burkholderia mallei infection, such as blood, sputum, urine, feces, local lesions and purulent exudate, as well as environmental contaminated specimens such as water samples and soil, for auxiliary diagnosis of Burkholderia mallei infection.
Specimen collection
The patient's blood, sputum, urine, feces, local lesions, and purulent exudate; Environmental pollution samples such as water samples and soil.
