Influenza A/Influenza B (IAV/IBV) Dual Nucleic Acid Detection Kit (PCR-Fluorescent Probe Method)
2025-03-19Paenibacillus mucilaginosus Probe-Based qPCR Kit
2025-03-19Product Number: DTK554
Shipping and Storage
- Transportation: The reagent kit must be transported under frozen conditions.
- Storage: Store at -20℃ and avoid repeated freezing and thawing.
- Validity period: 12 months, please use within the validity period.
Component
| Component | 50T |
| Nuclease-Free Water | 1000μL |
| 2×nucleic acid amplification reaction solution | 500μL |
| 20×reverse transcriptase | 50μL |
| 10×Primer Probe Reaction Solution (MP351Z) | 100μL |
| MP351Z - Positive control | 100μL |
| Negative control | 1000μL |
Note: Different batches of reagents cannot be mixed.
Description
This kit uses real-time fluorescence PCR technology and is suitable for detecting hepatitis A virus and hepatitis E virus nucleic acids extracted from feces. Each reaction system contains specific primers and fluorescent probes for detecting hepatitis A virus and hepatitis E virus. By collecting the fluorescent signals generated by PCR amplification, qualitative detection of the nucleic acid of hepatitis A virus and hepatitis E virus can be performed.
Application
This kit is suitable for qualitative detection of nucleic acids of Human Hepatitis A Virus (HAV) and Human Hepatitis E Virus (HEV) extracted from feces. The experimental results only provide reference for basic research and are not used as clinical diagnostic basis.
Human Hepatitis A virus (HAV) is a small RNA virus belonging to the Hepadnavirus family. HAV has a stable structure, high titer secretion, and can be effectively transmitted between humans and water sources through the fecal oral route. After infection, the virus can manifest as acute viral hepatitis. Hepatitis E virus (HEV) belongs to the hepatitis virus genus of the hepatitis virus family, and is an unencapsulated spherical particle. It has been found that four genotypes can infect humans. HEV is mainly transmitted through fecal oral route in developing countries, while in regions such as Western Europe and Japan, it is transmitted through direct ingestion of meat or organs from infected animals. HEV infection often presents as self limiting, but chronic infection may occur in patients treated with immunosuppressive agents, which has become a serious clinical problem in developed countries with a high incidence of organ transplantation cases.
Sample requirements
RNA samples extracted from feces can be tested using this kit.
