Product Number: DTK548
Shipping and Storage
Low temperature transportation, stored at -20℃, with a shelf life of one year. Positive controls need to be placed separately and should not contaminate other reagents.
Component
| Component | Specification |
| 2×OneStep Probe Mix | 600μL |
| OneStep Probe Enzyme Mix | 60μL |
| DEPC-H2O | 1mL |
| Rat SDHA RT-qPCR Primer Mix | 60μL |
| Rat SDHA RT-qPCR probe | 30μL |
| Rat SDHA RT-qPCR positive control (1 × 10E8 copy/μL) | 50μL |
Description
This kit can be used to detect the SDHA gene in rats. Succinate dehydrogenase flavon (SDHA), as the only active group in the SDH structure, is a key factor in electron transfer and respiratory function of coupled mitochondria.
Application
This product is a rat SDHA detection kit developed based on the principle of probe based fluorescence quantitative PCR. It has the following characteristics:
- Ready to use, users only need to provide RNA templates.
- Provide a positive control to distinguish false negative samples.
- Fast response and high sensitivity.
- This product is sufficient for 50 fluorescent quantitative RT-PCR reactions using a 20μL probe system.
- This product can only be used for scientific research.
Specimen collection
Sample RNA, ultrapure water.
