Mycobacterium leprae (ML) Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-03-19Donkey Nucleic Acid Detection Kit (Fluorescent PCR Method)
2025-03-19Product Number: DTK351
Shipping and Storage
- The reagent kit must be transported under frozen conditions.
- Store at -20 ℃ and avoid repeated freezing and thawing. The freezing and thawing times of the reagent kit shall not exceed 7 times.
Component
| Component | 50T |
| Nucleic acid amplification reaction mixture | 900μL |
| Enzyme solution | 100μL |
| Positive control for Rift Valley fever | 100μL |
| Negative control | 1000μL |
Note: Different batches of reagents cannot be mixed.
Description
This reagent uses real-time fluorescence PCR technology and is suitable for detecting Rift Valley fever virus nucleic acid extracted from whole blood, serum, and their cultures. Each reaction system contains specific primers and fluorescent probes for detecting the genes of Rift Valley fever virus. By collecting the fluorescent signals generated by PCR amplification, qualitative detection of Rift Valley fever virus nucleic acid can be performed.
In addition, specific primers and fluorescent probes for internal standard control of human clinical specimens were added to each reaction system to monitor the collection, transportation, and extraction process of the test samples, indicating false negatives in the detection results.
Application
This kit is suitable for qualitative detection of Rift Valley fever virus nucleic acid extracted from whole blood or serum and their cultures. The experimental results only provide reference for basic research and are not used as clinical diagnostic basis.
Rift Valley fever is an acute febrile animal disease caused by the Rift Valley fever virus. The virus is mainly transmitted by mosquitoes and can infect humans and animals. After infection, people usually experience symptoms such as fever, headache, bleeding, and shock.
Specimen collection
RNA samples extracted from whole blood, serum, and their cultures can all be tested using this kit testing.
Serum: After natural coagulation of blood without anticoagulants, extract the supernatant.
