2x Taq PCR Master Mix with blue dye
2021-09-01
2xSYBR qPCR Master Mix
2021-09-01Supplied by bulk package
Description of the 2x PCR master mix
This PCR master mix contains the Taq DNA Polymerase, dNTPs, and optimized buffer but without any dye in it. This Taq Mastermix is provided at 2× concentration and used at 1× concentration by adding the template, primer, and H2O. It has the 5′to 3′DNA polymerase activity and 5′to 3′exonuclease activity without 3′-5′exonuclease activity. The extending speed is 1-2 kb/min. There is an "A" on the 3′end. The PCR product can be cloned in a TA vector.
Quality Control
This product has passed the following quality control assays: functional absence of double- and single-stranded endonuclease activity; >90% homogeneous by SDS gel electrophoresis. Each lot of our Taq DNA Polymerase is assayed for amplification from as little as 10 ng of human genomic DNA
Application
- PCR
- Primer Extension
- Microarray Analysis
- High-Throughput PCR
Storage: Store at -20°C
Shipping with blue ice
Reaction Mixture Set Up
Template <0.5 μg
Forward Primer (10 μM) 1 μl
Reverse Primer (10 μM) 1 μl
2×TransTaq PCR SuperMix 25 μl
ddH2O to final volume 50 μl
No repeated freezing and thawing and split charging for good amplification.
