DTK146, Neospora spp. (Ne) Nucleic Acid Detection Kit (Fluorescent PCR Method). This kit uses TaqMan probe method for real-time fluorescence PCR technology, designs a pair of canine Neosporidium specific primers, combines with a specific probe, and uses fluorescence PCR technology to amplify and detect the DNA of canine Neosporidium in vitro, which is used for pathogenic diagnosis of suspected infectious materials in clinical practice.