The Hot Start Taq DNA Polymerase is a recombinant Taq DNA polymerase that has been chemically modified by the addition of heat-labile blocking groups to its amino acid residues.
The 2×TaqMan Master Mix is a special mixture for Probe real-time PCR(TaqMan, Molecular Beacon, etc.). Which include the HotStar DNA Polymerase, PCR Buffer, MgCl2, dNTPs, ROX, and the stabilizing agent.